Multiple Forms of Supernatant Malate Dehydrogenase in Salmonid Fishes

Multiple forms of supernatant malate dehydrogenase in salmonid fishes.

Salmon generally possess three forms of supernatant malate dehydrogenase whose subunit compositions are indicated by the formulas AA, AB, and BB. Each of the homodimers (AA and BB) has now been purified to homogeneity. The two enzymes are similar in molecular size and in catalytic properties to the supernatant malate dehydrogenases of higher vertebrates; both are catalytically distinct from the...

Malate dehydrogenase, inhibition of pig heart supernatant enzyme by iodoacetamide.

Pig heart supernatant malate dehydrogenase is alkylated by 250 mM iodoacetamide at 37 degrees C in pH 7.5 Tris/acetate buffer which is 0.05 M with respect to acetate to form enzyme with 1,3-dicarboxamidomethyl histidine, 3-carboxamidomethyl histidine, 1-carboxamidomethyl histidine, carboxamidomethyl cysteine, and carboxamidomethyl methionine. 1,3-Dicarboxamidomethyl histidine forms with a stoic...

Organelle and translocatable forms of glyoxysomal malate dehydrogenase

doi:10.1111/j.1742-4658.2004.04475.x Many organelle enzymes coded for by nuclear genes have N-terminal sequences, which directs them into the organelle (precursor) and are removed upon import (mature). The experiments described below characterize the differences between the precursor and mature forms of watermelon glyoxysomal malate dehydrogenase. Using recombinant protein methods, the precurso...

THE EFFECT OF SALT STRESS ON MALATE DEHYDROGENASE IN WHEAT

Effect of various NaCI treatments (0, 50, 100, 200 and 300 mM) at different growth and development stages (tillering, boot swollen, flowering and anthesis) of two wheat cultivars  on the kinetic activity and PAGE electrophoretic pattern of leaf malate dehydrogenase was studied under greenhouse conditions. Ghods was salt-sensitive and Boolani was salt-tolerant. In general, in response to salinti...

Proliferative Kidney Disease in Salmonid Fishes